| Intracellular osteopontin potentiates the immunosuppressive activity of mesenchymal stromal cells |
| 論文作者 |
Yang, WL; Jin, M; Gu, YT; Zhao, XA; Zhu, LQ; He, S; Wang, H; Ding, XY; Wang, B; Jiang, TW; Xiao, YC; Zhou, GQ; Huang, JF; Zhang, YY |
| 期刊/會議名稱 |
STEM CELL RESEARCH & THERAPY |
| 論文年度 |
2024 |
| 論文類別 |
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| 摘要 |
IntroductionMesenchymal stromal cell (MSC)-based cell therapy is a promising approach for various inflammatory disorders based on their immunosuppressive capacity. Osteopontin (OPN) regulates several cellular functions including tissue repair, bone metabolism and immune reaction. However, the biological function of OPN in regulating the immunosuppressive capacity of MSCs remains elusive.ObjectivesThis study aims to highlight the underlying mechanism of the proinflammatory cytokines affect the therapeutic ability of MSCs through OPN.MethodsMSCs in response to the proinflammatory cytokines were collected to determine the expression profile of OPN. In vitro T-cell proliferation assays and gene editing were performed to check the role and mechanisms of OPN in regulating the immunosuppressive capacity of MSCs. Inflammatory disease mouse models were established to evaluate the effect of OPN on improving MSC-based immunotherapy.ResultsWe observed that OPN, including its two isoforms iOPN and sOPN, was downregulated in MSCs upon proinflammatory cytokine stimulation. Interestingly, iOPN, but not sOPN, greatly enhanced the immunosuppressive activity of MSCs on T-cell proliferation and thus alleviated the inflammatory pathologies of hepatitis and colitis. Mechanistically, iOPN interacted with STAT1 and mediated its deubiquitination, thereby inducing the master immunosuppressive mediator inducible nitric oxide synthase (iNOS) in MSCs. In addition, iOPN expression was directly downregulated by activated STAT1, which formed a negative feedback loop to restrain MSC immunosuppressive capacity.ConclusionOur findings demonstrated that iOPN expression modulation in MSCs is a novel strategy to improve MSC-based immunotherapy. |
| 期 |
1 |
| 卷 |
15 |
| 影響因子 |
7.1 |
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